Journal article

Increased FOXJ1 protein expression is associated with improved overall survival in high-grade serous ovarian carcinoma: an Ovarian Tumor Tissue Analysis Consortium Study

Ashley Weir, Eun-Young Kang, Nicola S Meagher, Gregg S Nelson, Prafull Ghatage, Cheng-Han Lee, Marjorie J Riggan, Aleksandra Gentry-Maharaj, Andy Ryan, Naveena Singh, Martin Widschwendter, Jennifer Alsop, Michael S Anglesio, Matthias W Beckmann, Jessica Berger, Christiani Bisinotto, Jessica Boros, Alison H Brand, James D Brenton, Angela Brooks-Wilson Show all

BRITISH JOURNAL OF CANCER | SPRINGERNATURE | Published : 2023

Abstract

Background: Recently, we showed a >60% difference in 5-year survival for patients with tubo-ovarian high-grade serous carcinoma (HGSC) when stratified by a 101-gene mRNA expression prognostic signature. Given the varied patient outcomes, this study aimed to translate prognostic mRNA markers into protein expression assays by immunohistochemistry and validate their survival association in HGSC. Methods: Two prognostic genes, FOXJ1 and GMNN, were selected based on high-quality antibodies, correlation with protein expression and variation in immunohistochemical scores in a preliminary cohort (n = 134 and n = 80, respectively). Six thousand four hundred and thirty-four (FOXJ1) and 5470 (GMNN) for..

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University of Melbourne Researchers

Grants

Awarded by NCI/NIH


Awarded by NIHR Cambridge Biomedical Research Centre


Awarded by U.S. Army Medical Research and Materiel Command


Awarded by National Health and Medical Research Council of Australia (NHMRC)


Awarded by AOV: Canadian Institutes of Health Research


Awarded by BRZ: Brazilian National Council for Scientific and Technological Development


Awarded by CAL: Cancer Research Society


Awarded by CLS Internal Research


Awarded by Instituto de Salud Carlos Tercero


Awarded by Instituto de Salud Carlos III


Awarded by Ministerio de Economia y Competitividad


Awarded by DOV: NCI/NIH


Awarded by HAW: US National Institutes of Health


Awarded by HOP: Department of Defense


Awarded by NCI


Awarded by LAX: American Cancer Society Early Detection


Awarded by National Center for Advancing Translational Sciences (NCATS)


Awarded by MAY: National Institutes of Health


Awarded by SEA: Cancer Research UK


Awarded by Canadian Institutes of Health Research grant


Awarded by NIH/NCI


Awarded by Eve Appeal (The Oak Foundation)


Awarded by WMH: National Health and Medical Research Council of Australia


Awarded by Cancer Institute NSW


Awarded by National Health and Medical Research Council of Australia (NHMRC Fellowship


Awarded by United States Department of Defence (DoD) Ovarian Cancer Transitional Leverage Award


Awarded by Brazilian National Council for Scientific and Technological Development -CNPq


Awarded by Calgary Laboratory Services


Awarded by American Cancer Society Early Detection Professorship


Awarded by Cancer Research UK


Awarded by European Union



Funding Acknowledgements

[ "This study was funded by NCI/NIH R01CA172404 to SJR. This research was supported by the NIHR Cambridge Biomedical Research Centre (BRC-1215-20014). The views expressed are those of the authors and not necessarily those of the NIHR or the Department of Health and Social Care. AOC: The Australian Ovarian Cancer Study was supported by the U.S. Army Medical Research and Materiel Command under DAMD17-01-1-0729, The Cancer Council Victoria, Queensland Cancer Fund, The Cancer Council New South Wales, The Cancer Council South Australia, The Cancer Foundation of Western Australia, The Cancer Council Tasmania and the National Health and Medical Research Council of Australia (NHMRC; ID199600; ID400413 and ID400281); AOV: Canadian Institutes of Health Research (MOP-86727).; BAV: ELAN Funds of the University of Erlangen-Nuremberg; BRZ: Brazilian National Council for Scientific and Technological Development, grant No. 478416/2009-1; CAL: Cancer Research Society (19319) and CLS Internal Research support RS14-5008; CNI: CNI funded by Instituto de Salud Carlos Tercero (AES grant PI19/01730) and Fondo Europeo de Desarrollo Regional, FEDER. Instituto de Salud Carlos III (PI 12/01319); Ministerio de Economia y Competitividad (SAF2012); DOV: NCI/NIH R01CA168758, NCI/NIH R01CA112523 and NCI/NIH R01CA087538; HAW: US National Institutes of Health (R01-CA58598, N01-CN-55424 and N01-PC-67001); HOP: Department of Defense (DAMD17-02-1-0669) and NCI (K07-CA080668, R01-CA95023, MO1RR000056). This project used the UPMC Hillman Cancer Center and Tissue and Research Pathology/Pitt Biospecimen Core shared resource which is supported in part by award P30CA047904. LAX: American Cancer Society Early Detection Professorship (SIOP-06-258-01-COUN) and the National Center for Advancing Translational Sciences (NCATS), Grant UL1TR000124; MAY: National Institutes of Health (R01-CA122443, R01-CA243483, P30-CA15083, P50-CA136393); Mayo Foundation; Minnesota Ovarian Cancer Alliance; Fred C. and Katherine B. Andersen Foundation; NCT: Parts of the study at the NCT were funded by \"Heuer Stiftung fur medizinische Forschung\"; POC: Pomeranian Medical University; SEA: Cancer Research UK C490/A16561, the UK National Institute for Health Research Biomedical Research Centres at the University of Cambridge, Cambridge Cancer Centre. The University of Cambridge has received salary support for PDPP from the NHS in the East of England through the Clinical Academic Reserve. TVA: This work was supported by Canadian Institutes of Health Research grant (MOP-86727) and by NIH/NCI 1 R01CA16066901A1; UKO: The UKOPS study was funded by The Eve Appeal (The Oak Foundation) with contribution to author's salary through MRC core funding MC_UU_00004/01 and the National Institute for Health Research University College London Hospitals Biomedical Research Centre; VAN: BC's Gynecological Cancer Research Team (OVCARE) receives core funding from The BC Cancer Foundation and the VGH and UBC Hospital Foundation; WMH: National Health and Medical Research Council of Australia, Enabling Grants ID 310670 and ID 628903. Cancer Institute NSW Grants 12/RIG/1-17 and 15/RIG/1-16. The Westmead GynBiobank acknowledges financial support from the Sydney West Translational Cancer Research Centre, funded by the Cancer Institute NSW. NSM is supported by the NSW Ministry of Health and UNSW Sydney under the NSW Health PhD Scholarship Program, and the Translational Cancer Research Network, a translational cancer research centre programme funded by the Cancer Institute NSW.", "DB is supported by the National Health and Medical Research Council of Australia (NHMRC Fellowship (APP1117044) and Program Grant APP1092856) and the United States Department of Defence (DoD) Ovarian Cancer Transitional Leverage Award (W81XWH-12-1-0104); FJCdR is funded by the Brazilian National Council for Scientific and Technological Development -CNPq (427983/20169, 303210/2018-4). MK received support through the Calgary Laboratory Services research support fund (RS19-612). AB received support through the Ovarian Cancer Research Fund (OCRF). BYK is funded by the American Cancer Society Early Detection Professorship (SIOP-06-258-01-COUN) and the National Center for Advancing Translational Sciences (NCATS), grant UL1TR000124. SH received grants from University of Heidelberg Medical Faculty, Novartis Oncology, Stiftung fur Krebsund Scharlachforschung Mannheim, and Deutsche Forschungsgemeinschaft. JDB acknowledges funding and support from Cancer Research UK (grant numbers A22905, A15601 and A17197). UKO: MW is funded by the European Union's Horizon 2020 European Research Council Program, H2020 BRCA-ERC under Grant Agreement No. 742432. AT is funded through a Michael Smith Foundation for Health Research Scholar Award. MA is funded through a Michael Smith Foundation for Health Research Scholar Award and the Janet D. Cottrelle Foundation Scholars programme managed by the BC Cancer Foundation. DGH receives support from the Dr. Chew Wei Memorial Professorship in Gynecologic Oncology and the Canada Research Chairs programme (Research Chair in Molecular and Genomic Pathology). Funding for ADF is funded by Cancer Institute NSW grant 15/TRC/1-01. RE was supported by the Interdisciplinary Center for Clinical Research (IZKF; Clinician Scientist Program) of the Medical Faculty FAU Erlangen-Nurnberg. The funding sources had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript and decision to submit the manuscript for publication. Open Access funding enabled and organized by CAUL and its Member Institutions." ]